Isolation of hen's egg phosphonolipids by thin-layer chromatography, their identification and silicic acid column chromatographic separation.

The isolation and identification of the phosphonolipids from beef brain--right lobe, left lobe and cerebellum--is herein reported. The phosphonolipids were isolated by preparative thin layer chromatography (TLC) using the solvent system methanol/water (2:1, v/v) and identified preliminarily by TLC. The isolated phosphonolipids were subjected to silicic acid column chromatographic separation and the thus separated phosphonolipids were identified by TLC, nitrogen-phosphorus determinations and IR spectroscopy.